Synthesis of Silver Nanoparticles Using Dichloromethane-Methanol Flower Extract of Chrysanthemum cinerariaefolium and Its Antibacterial Activity

Abstract

Nanotechnology is an emerging field that has opened new horizons in nanomedicine. The use of silver nanoparticles is attracting much interest because of their antibacterial activity. This study involved synthesis of silver nanoparticles using Chrysanthemum cinerariaefolium flowers dichloromethane-methanol crude extract. The synthesized silver nanoparticles (Ag NPs) were characterized using UV-Vis spectroscopy, SEM, EDX, TEM and FTIR. The antibacterial potential of the nanoparticles was ascertained against methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, Staphylococcus aureus and Shigella sonnei. This was followed by phytochemical analyses of the crude extracts. The Ag NPs were generally spherical as observed in the SEM and TEM micrographs with an average size of 26.98 nm. The UV- absorption spectrum revealed prominent peak at 430 nm while EDX analysis showed the percentage abundance of silver nanoparticle at (81.33%). The FTIR spectroscopy confirmed absorption bands of various functional groups on the surface of Ag NPs. The absorption bands were at 3472.88 cm-1, 3190.67 cm-1, 1646.61 cm-1, 1405.08 cm-1, 1109.32 cm-1 and 518.64 cm-1. Antibacterial potential of the synthesized Ag NPs showed that they were more active on S. aureus with an MIC of 31.25 µg/ml. The phytochemicals observed in the crude extracts that could have been responsible for reducing silver ions into silver nanoparticles were flavonoids, phenols, tannins and glycosides.