Platinum test in biological objects

Abstract

At the problematic lab of the Seversk Technological Institute of Tomsk Polytechnic University (STI TPU) two devices are used for heavy metals testing in biological objects: an analyzer (TA-1) which uses the method of inverse voltammetry (IV), and a spectrometer (Spectroskan) which uses the method of X-ray-fluorescence analysis (XFA). Professor N.A. Rolpakova (TPU, Chemical Engineering Faculty) uses the technique of platinum (Pt) test in biological materials (blood, urine, tissue) of patients having Pt treatment by an IV method was developed. Pt is used for treatment of oncological diseases. Its molecules bind the cells of a cancerous growth. To know the concentration of this drug in the blood of an oncological patient is necessary for overseeing a course of treatment. The essence of the method is the following: the Pt from assay is transferred to solution. The solution is placed in glass for polarographic measurement. The electrodes are inserted: an operating electrode (impregnated graphite electrode) and a comparison electrode (AgCl). During a definite time (the time of electrolysis) the Pt concentrates on the surface of an electrode together with mercury, which is beforehand entered in a parsed solution (metal-activator). The current of oxidation of the metal-activator, deposited together with Pt, is proportional to the content of Pt in the parsed assay. The content of Pt is determined by a method of standard components.