Protective Effect of Angelica sinensis Extract and Origanum majorana Oil on Hepatic and Renal Toxicities Induced by Nickel Chloride in Male Albino Rats

Zagazig Veterinary Journal Pub Date : 2019-09-01 DOI:10.21608/zvjz.2019.13886.1050

M. Hashem, H. Ismail, E. Hassan

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引用次数: 2

Abstract

The present study was accomplished to evaluate the efficacy of Angelica sinensis root extract and Origanum majorana oil on subchronic toxicity of nickel chloride (NiCl2) in albino rats by studying the changes in the biochemical parameters, lipid peroxidation and antioxidant status related to the liver and kidneys. This study was carried out on sixty male albino rats with average body weight (120 ± 10 g), which were divided into 6 equal groups. Gp.(1): were kept as control on distilled water, rats in Gp. (2) received NiCl2 (0.75 mg/kg BW), Gp. (3) received A. sinensis root extract (300 mg/kg BW) and Gp. (4) received O. majorana oil (0.5 ml/kg BW). NiCl2 intoxicated rats in Gp. (5) and Gp. (6) were administered A. sinensis root extract and O. majorana oil at the same former doses, respectively. The treatments were daily and orally for 4 weeks. Blood and tissue samples were collected for performing the different experimental analysis after 4 weeks from starting the treatments. Comparing with the control group, results showed that NiCl2 significantly increased serum alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase activities and serum bilirubin, triglycerides, total cholesterol, urea and creatinine concentrations, besides hepatic and renal malondialdehyde concentration, while serum total proteins, albumin and hepatic and renal glutathione, glutathione peroxidase, superoxide dismutase and catalase levels were significantly decreased. A. sinensis root extract and O. majorana oil has no adverse effects and worked on amelioration the various alterations in estimated analytes in combined treated groups. In conclusion, NiCl2 caused hepatic and renal injuries besides the induction of lipid peroxidation and reducing the antioxidant enzymes activities in these organs. Moreover, both of A. sinensis root extract and O. majorana oil provided significant protection against harmful effects of NiCl2 with superiorty of A. sinensis root extract

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当归提取物和大黄油对氯化镍致雄性白化大鼠肝、肾毒性的保护作用

本研究通过对白化大鼠肝、肾相关生化指标、脂质过氧化和抗氧化状态的变化，评价当归根提取物和大黄芷油对氯化镍(NiCl2)亚慢性毒性的影响。实验选用平均体重(120±10 g)的雄性白化大鼠60只，随机分为6组。Gp.(1):以蒸馏水为对照，Gp大鼠为对照。(2)给予NiCl2 (0.75 mg/kg BW)、Gp;(3)分别给予三叶草根提取物(300 mg/kg BW)和Gp。(4)注射大叶豆油(0.5 ml/kg BW)。Gp中NiCl2中毒大鼠。(5)和Gp。(6)分别给药相同剂量的中华黄芪根提取物和黄芪油。治疗方法为每日口服，连续4周。治疗开始4周后，收集血液和组织样本进行不同的实验分析。结果表明，与对照组相比，NiCl2显著提高了血清丙氨酸转氨酶、天冬氨酸转氨酶和碱性磷酸酶活性，显著提高了血清胆红素、甘油三酯、总胆固醇、尿素和肌酐浓度，显著降低了肝脏和肾脏丙二醛浓度，显著降低了血清总蛋白、白蛋白和肝脏和肾脏谷胱甘肽、谷胱甘肽过氧化物酶、超氧化物歧化酶和过氧化氢酶水平。在联合处理组中，三叶草根提取物和三叶草油对估计分析物的各种变化没有不良影响，并有改善作用。综上所述，NiCl2除了诱导脂质过氧化和降低这些器官的抗氧化酶活性外，还引起肝脏和肾脏的损伤。结果表明，木香根提取物和木香油对NiCl2的有害作用均有显著的保护作用，且木香根提取物具有明显的保护作用

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Zagazig Veterinary Journal

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