Automatic segmentation and counting of Skeletonema costatum obtained by flow cytometry image

Wang Di, Xie Jiezhen
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引用次数: 0

Abstract

Skeletonema costatum (S. costatum) is one of the most common species that can give rise to red tide, a global marine disaster. For the purpose to monitor and forecast the outburst of red tide caused by S. costatum, many measures have been brought up. In this paper we propose a recursive method to segment and calculate S. costatum images captured by our improved Flow Cytometry. Firstly Otsu method is applied to separate the algae cells from the background. Afterward, the flood filling algorithm is adopted to fill the holes produced by the Otsu method. Then we use the erosion procedure to recursively find out the contours of each S. costatum cells. The result shows that our method can correctly separate most of the cells.
流式细胞术图像对肋骨的自动分割与计数
骨骸藻(S. costatum)是最常见的物种之一,可以引起红潮,一种全球性的海洋灾难。为了监测和预报海螺引起的赤潮爆发,提出了许多措施。在本文中,我们提出了一种递归的方法来分割和计算我们改进的流式细胞术捕获的鱼形纹图像。首先采用Otsu方法从背景中分离藻类细胞。然后,采用洪水填充算法对Otsu法产生的孔洞进行填充。然后,我们使用侵蚀程序递归地找出每个蛇形骨细胞的轮廓。结果表明,该方法可以正确地分离大部分细胞。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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