Design of a New Bioluminescence Sensor Using an Arduino Device Linked to Bluetooth Mobile Phone and USB Computer to Measure Triglycerides

Ammar Mohammed Ali, M. A. Kadhem
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Abstract

In this work, a new device is designed for measuring the light intensity of the Bioluminescence reaction between Luminol with Hydrogen peroxide that is produced as an activity of Triglycerides Phosphate Oxidase. The project includes the use of a homemade and semi-automated device for the determination of Chemiluminescence (CL) and Bioluminescence light by direct reaction analysis of Luminol with oxidant and using a Mobile –phone as a recorder which is employed in chemistry with Arduino and applied this device into the enzymatic reaction for determine Triglyceride by this new methods. The method is easy, simple, and rapid with high sensitivity for the determination of hydrogen peroxide. The light of the Bioluminescence reaction of Luminol (5-amino-2,3-dihydro-1,4_phthalazinedione) with oxidant (H2O2) was sensed by photocell and the signal was sent to Mobile. The method used to determine glycerol and compare the results of the device with real value to give high accuracy, Triglyceride was determined in its kit. It gave 99.2 and ±0.1 of Recovery and RSD, respectively.
一种新型生物发光传感器的设计,使用Arduino设备连接蓝牙手机和USB计算机来测量甘油三酯
在这项工作中,设计了一种新的装置,用于测量鲁米诺与过氧化氢之间的生物发光反应的光强度,过氧化氢是甘油三酯磷酸氧化酶的活性产生的。本项目采用自制的半自动化装置,通过对鲁米诺与氧化剂的直接反应分析来测定化学发光(CL)和生物发光(Bioluminescence)光,并使用Arduino作为化学中的手机作为记录仪,将该装置应用于酶法测定甘油三酯的新方法。该方法简便、快速、灵敏度高,可用于过氧化氢的测定。利用光电池检测鲁米诺(5-氨基-2,3-二氢-1,4 -酞嗪二酮)与氧化剂(H2O2)生物发光反应的光,并将信号发送到移动设备。该方法用于测定甘油,并将装置的结果与实际值进行比较,以获得较高的准确性,在其试剂盒中测定甘油三酯。回收率为99.2,RSD为±0.1。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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