Electrophoretic Banding Pattern Of Esterase Isozyme In Different Tissues Of Puntius Sophore (Cyprinidae : Cypriniformes)

H. Jahan, Partha Sarathi Gope, M. S. Alam, R. Shahjahan
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Abstract

Esterase isozyme of different tissues of Puntius sophore was analyzed using 7.5 % polyacrylamide gel electrophoresis (PAGE). Fish specimens were collected from local market. The tissues used were taken from different muscles, stomach, fore-, mid- and hind-gut, liver, eyes, gill, heart, kidney, fore-, mid- and hind- brain, eggs and ovary. Six different esterase bands were detected, named Est-1, Est-2, Est-3, Est-4, Est-5 and Est-6 and their relative mobility were 1.0, 0.84, 0.62, 0.33, 0.26 and 0.13 respectively, each of them representing a single allele. The highest esterase activity was found in liver, followed by gill, kidney, heart, brain, intestine, stomach, eye, reproductive organ and skeletal muscles as detected in the staining intensity. Staining intensity of Est-4 and Est-5 was higher and Est-6 was the least stained in all the tissues. Asiat. Soc. Bangladesh, Sci. 42(2): 201-208, December 2016
大花扁豆不同组织中酯酶同工酶的电泳带型
采用7.5%聚丙烯酰胺凝胶电泳(PAGE)技术对大蓬不同组织的酯酶同工酶进行了分析。鱼类样本在本地市场采集。所使用的组织取自不同的肌肉、胃、前、中、后肠、肝脏、眼睛、鳃、心脏、肾脏、前、中、后脑、卵子和卵巢。检测到6条不同的酯酶条带,分别为Est-1、Est-2、Est-3、Est-4、Est-5和Est-6,它们的相对迁移率分别为1.0、0.84、0.62、0.33、0.26和0.13,每条条带代表一个等位基因。肝脏中酯酶活性最高,其次为鳃、肾、心、脑、肠、胃、眼、生殖器官和骨骼肌。所有组织中Est-4和Est-5染色强度较高,Est-6染色最少。Asiat。Soc。中国科学,42(2):201-208,2016年12月
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