Detection Limit of Surface Plasmon Resonance Sensor for Quantitative Foodborne E.coli Detection Using Effective Refractive Index Theory : The theoretical limit of E.coli detection of surface plasmon resonance

Nattha Pensupa, T. Treebupachatsakul, Chanin Lochotinunt, Suejit Pechprasarn
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Abstract

Surface plasmon resonance has been a gold standard for label-free biomedical and biochemical measurements, such as protein binding kinetics, protein-protein interactions. The surface plasmon resonance has also been utilized in food safety screening, including Escherichia coli and Salmonella detection. The theory of surface plasmon resonance has been well established and demonstrated its ultra-sensitivity to detect small nucleotides, proteins, and molecules. However, the results, so far, for the E.coli detection under the surface plasmon resonance have not shown an impressive detection limit. The typical detection limit of $E. c o l i$ under the conventional surface plasmon detection platform is around $10^{3} \mathrm{CFU} / \mathrm{ml}$. The detection limit can be enhanced using a secondary binding agent to E.coli, including conjugated nanoparticles. Here, we propose a theoretical framework using effective refractive index theory to explain the detection mechanism and give an insight into the underlining obstacles that degrades the detection limit of the surface plasmon resonance.
利用有效折射率理论定量检测食源性大肠杆菌表面等离子体共振传感器的检出限:表面等离子体共振检测大肠杆菌的理论限
表面等离子体共振已成为无标记生物医学和生化测量的金标准,如蛋白质结合动力学,蛋白质-蛋白质相互作用。表面等离子体共振也被用于食品安全筛查,包括大肠杆菌和沙门氏菌的检测。表面等离子体共振理论已经很好地建立,并证明了其超灵敏度检测小核苷酸,蛋白质和分子。然而,到目前为止,在表面等离子体共振下检测大肠杆菌的结果还没有显示出令人印象深刻的检测极限。典型的检出限为$E。传统表面等离子体探测平台下的co l i$约为$10^{3}\ mathm {CFU} / \ mathm {ml}$。利用大肠杆菌的二级结合剂(包括共轭纳米颗粒)可以提高检测限。在这里,我们提出了一个理论框架,利用有效折射率理论来解释检测机制,并深入了解降低表面等离子体共振检测极限的潜在障碍。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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