The efficacy and mechanism of fecal microflora transplantation in the treatment of slow transit constipation based on metabonomics anion mode

Abstract

Background: Fecal microbiota transplantation (FMT) based on the positive ion mode of metabonomics has a good therapeutic benefit for slow transit constipation (STC) patients. However, a piece of comprehensive metabolomics information is yet to be established. The aim of the study was to explore the efficacy and mechanism of FMT in the treatment of STC under metabonomics. Methods: Eight STC patients meeting the set inclusion and exclusion criteria were enrolled and treated with FMT (three times). The Patient Assessment of Constipation-Symptoms (PAC-SYM), weekly total defecation times, and defecation frequency scores of these STC patients were compared before and after treatment. Feces and serum of STC patients before and after treatment were analyzed using 16SrDNA and metabolomics. Results: After FMT treatment, the PAC-SYM score of constipated patients decreased [(5.00 ± 2.94) vs (5.20 ± 2.87)], while the number of complete defecations per week increased [(2.00 ± 1.79) vs (1.69 ± 1.80)]. The score of defecation frequency decreased [(0.83 ± 1.03) vs (0.86 ± 0.95)]. The metabolites in the feces and serum of patients receiving FMT changed significantly ( P < 0.05). The results from 16SrDNA analysis showed that the α and β diversity of the fecal microbiome changed significantly ( P < 0.05) after transplantation, and the contents of genera Lactobacillus , Bacillus , Succiniclasticum , Cellvibrio , and Escherichia increased in FMT treated patients. Conclusion: FMT may treat STC by increasing the beneficial intestinal flora and metabolites in the anion mode of metabolomics.