The role of fibrin as a ligand for ICAM-1 and PMN in melanoma adhesion under different shear conditions.

2009 14th National Biomedical Engineering Meeting Pub Date : 2009-05-20 DOI:10.1109/BIYOMUT.2009.5130385

Tugba Ozdemir, C. Dong

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Abstract

One of the predominant cause of cancer patient death is the metastatic spread of tumor cells. The leading step in melanoma extravasation is the tumor cell adhesion to the endothelium of the capillaries. However, melanoma cells do not express the appropriate adhesion molecules to bind to an activated endothelium. Previous studies have found neutrophils facilitate melanoma cell adhesion under dynamic flow conditions throught the binding of ICAM-1 on melanoma cells and β2 integrins on PMNs. In addition, soluble fibrin (sFn) is a marker for propagated intravascular coagulation and have a dramatical increase in cancer patients' laboratory coagulation inspections . Soluble fibrin molecule has both receptors for Mac-1 on PMNs and ICAM-1 on tumor cell surface which may affect the tumor cell adherence to endothelial wall surface via PMN. The objective of this study is to investigate the role of soluble fibrin on PMN facilitated tumor cell adherence to endothelial wall and how different effects of hydrodynamic shear stress and shear rate affects the force of adhesion to vessel wall.

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纤维蛋白作为ICAM-1和PMN的配体在不同剪切条件下黑色素瘤粘附中的作用。

肿瘤细胞的转移扩散是癌症患者死亡的主要原因之一。黑色素瘤外渗的主要步骤是肿瘤细胞粘附到毛细血管内皮。然而，黑色素瘤细胞不表达适当的粘附分子来结合活化的内皮细胞。先前的研究发现，中性粒细胞通过ICAM-1结合黑色素瘤细胞和β2整合素结合PMNs，促进黑色素瘤细胞在动态流动条件下的粘附。此外，可溶性纤维蛋白(sFn)是血管内扩散性凝血的标志物，在癌症患者的实验室凝血检查中有显著增加。可溶性纤维蛋白分子在PMN上有Mac-1受体，在肿瘤细胞表面有ICAM-1受体，可能通过PMN影响肿瘤细胞对内皮壁的粘附。本研究的目的是探讨可溶性纤维蛋白在PMN促进肿瘤细胞粘附内皮壁中的作用，以及不同流体动力剪切应力和剪切速率对肿瘤细胞粘附血管壁力的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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2009 14th National Biomedical Engineering Meeting

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