Optimization of Injection Conditions in Preparative Liquid Chromatography

Abstract

Abstract This paper describes the effect of injection volume on injectable sample load consistent with purity and recovery ratio of the solute of interest for different shapes of solute isotherms (convex or concave, without or with interactions between two adjacent solute bands). In preparative liquid chromatography, the column mass overloading conditions are usually thought to be better than volume overload conditions; according to this study, this statement is shown not to be true in all cases because optimization of injection conditions is much more complicated. For some isotherm shapes, there is an optimal injection volume in which injectable sample load is maximum.