Incorporation of radioiodinated IPPA and BMIPP fatty acid analogues into complex lipids from isolated rat hearts

Abstract

Heart lipids were extracted by the Folch technique from Langendorff-perfused rat hearts after administration of 15-(p-[¹³¹I]iodophenyl)pentadecanoic acid and 15-(p-[¹²⁵I]iodophenyl)-3-R,S-methylpentadecanoic acid. Techniques utilizing successive high performance liquid chromatographic (HPLC) analyses have been developed for the evaluation of the uptake of the tracers into neutral lipids and phospholipids of the rat hearts. Phospholipids were separated on a SiO₂ column eluted with a gradient of acetonitrile/water ($\text{97.5}\text{2.5}$) and acetonitrile/water ($\text{85}\text{15}$) followed by separation of the neutral lipids on a C-18 reversed phase column with a gradient consisting of acetonitrile and 2-propanol/hexane ($\text{60}\text{40}$) containing 1 N H₂SO₄ (5μL/100 mL). Both tracers show the incorporation into the expected major lipid classes.