SEQUENCE ANALYSIS OF siet gene IN STAPHYLOCOCCUS PSEUDINTERMEDIUS ISOLATEDFROM CANINE PYODERMA

Abstract

Astudy was conducted on isolation, identification and characterization of staphylococcal organisms associated with the canine pyoderma. The bacteriological processing of the samples resulted in the recovery of 95 staphylococcal isolates and 18 other bacterial isolates. On culture, staphylococci were the most predominantly (n=95, 75.39%) isolated organisms. Based on nucgene-based PCR, out of 95 staphylococcal isolates obtained, 82 (86.1%) of the isolates were found belonging to S. pseudintermedius. And out of 82 S. pseudintermedius isolates, siet gene was detected in 69 (86.1%) isolates. S. pseudintermedius was found to be predominant bacterial pathogen responsible for pyoderma in dogs. Two PCR products which were amplified for nuc gene and one each of siet and mec A gene were sent for commercial sequencing and the sequence alignment revealed 99.23 per cent, 99.14 per cent, 98.98 per cent and 100 per cent, similarities with the corresponding genes of S. pseudintermedius respectively.