Association of IL1B -511T>C, IL6 -634G>C, IL-6 -174G > C, miR -149 T>C AND miR-27aA>G gene polymorphisms with recurrent pregnancy loss risk in greek population

Abstract

Introduction: Recurrent pregnancy loss (RPL) is defined as the spontaneous loss of two or more idiopathic consecutive clinical pregnancies prior to 22 completed weeks of gestation. Association of miRNA polymorphisms with RPL has been investigated as a genetic determinant for the risk of idiopathic RPL. Moreover, mutations in several interleukins’ genes have been also related to RPL. Material and Methods: The study was carried out between September 2019 and September 2021 in Alexandra Maternity Hospital. Two hundred women with at least two consecutive spontaneous abortions (RPL) and 200 women as a control group who have completed one pregnancy were included in this study. Blood samples were collected from which genomic DNA was extracted, and PCR was performed to identify the IL1B -511T>C, IL6 -634G>C, IL-6 -174G > C, miR -149 T>C AND miR-27aA>G genotypes. Results: Of the 5 genes tested, genotype and group correlation were found in IL-1b-511 and miR-27. In specific, in the IL-1b-511 gene, cases have a 77.2% statistically significantly higher relative probability of having the polymorphism (CC genotype) than the control group (OR = 1.772, p = 0.007). In addition, in the miR-27 gene, cases are 3.2 times statistically significantly more likely to have polymorphism (GG) compared to controls (OR = 3.283, p <0.001). Conclusions: A genetic panel related to recurrent abortions is proposed. Gynecologists and researchers may apply it to establish a personalized treatment in women with RPL. The establishment of genetic panels related to infertility issues leads to more efficient solutions for the achievement of a pregnancy.