Analysis of Label-Free Single-Molecule Biosensors based on Gate-Biofunctionalized Organic Transistors

F. Torricelli, E. Macchia, K. Manoli, C. Franco, Z. Kovács-Vajna, G. Palazzo, G. Scamarcio, L. Torsi
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引用次数: 1

Abstract

A label-free single-molecule detection platform based on biofunctionalized organic transistors has been recently proposed. In contrast to state-of-art approaches based on nano-transducers, the aforementioned single-molecule transistor (SiMoT) sensing technology is based on millimeter-sized transistors where the gate is bio-functionalized with about a trillion of antibodies. In this work we provide a detailed investigation of SiMoT biosensors by studying and quantifying the device parameters affected by the biorecognition event. The study combines both measurements and theoretical analyses, showing that a single protein detection taking place at nanometer scale affects a millimeter scale area of the biofunctionalized gate electrode. The dimension of the bioprobe domains affected by the binding is calculated as a function of the nominal protein concentration in the analyte solution. These results provide important insight into the SiMoT biosensor technology, which can guide the development of multi-modal SiMoT detection and integrated SiMoT biosensors arrays.
基于栅极生物功能化有机晶体管的无标签单分子生物传感器分析
最近提出了一种基于生物功能化有机晶体管的无标签单分子检测平台。与基于纳米换能器的最先进方法相比,上述单分子晶体管(SiMoT)传感技术基于毫米大小的晶体管,其中栅极由大约一万亿抗体生物功能化。在这项工作中,我们通过研究和量化受生物识别事件影响的器件参数,对SiMoT生物传感器进行了详细的研究。该研究结合了测量和理论分析,表明在纳米尺度上进行的单个蛋白质检测会影响生物功能栅极的毫米尺度区域。受结合影响的生物探针结构域的尺寸被计算为分析溶液中名义蛋白质浓度的函数。这些结果为SiMoT生物传感器技术提供了重要的见解,可以指导多模态SiMoT检测和集成SiMoT生物传感器阵列的发展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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