F. Torricelli, E. Macchia, K. Manoli, C. Franco, Z. Kovács-Vajna, G. Palazzo, G. Scamarcio, L. Torsi
{"title":"Analysis of Label-Free Single-Molecule Biosensors based on Gate-Biofunctionalized Organic Transistors","authors":"F. Torricelli, E. Macchia, K. Manoli, C. Franco, Z. Kovács-Vajna, G. Palazzo, G. Scamarcio, L. Torsi","doi":"10.1109/IWASI.2019.8791380","DOIUrl":null,"url":null,"abstract":"A label-free single-molecule detection platform based on biofunctionalized organic transistors has been recently proposed. In contrast to state-of-art approaches based on nano-transducers, the aforementioned single-molecule transistor (SiMoT) sensing technology is based on millimeter-sized transistors where the gate is bio-functionalized with about a trillion of antibodies. In this work we provide a detailed investigation of SiMoT biosensors by studying and quantifying the device parameters affected by the biorecognition event. The study combines both measurements and theoretical analyses, showing that a single protein detection taking place at nanometer scale affects a millimeter scale area of the biofunctionalized gate electrode. The dimension of the bioprobe domains affected by the binding is calculated as a function of the nominal protein concentration in the analyte solution. These results provide important insight into the SiMoT biosensor technology, which can guide the development of multi-modal SiMoT detection and integrated SiMoT biosensors arrays.","PeriodicalId":330672,"journal":{"name":"2019 IEEE 8th International Workshop on Advances in Sensors and Interfaces (IWASI)","volume":"23 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2019-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"2019 IEEE 8th International Workshop on Advances in Sensors and Interfaces (IWASI)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/IWASI.2019.8791380","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
A label-free single-molecule detection platform based on biofunctionalized organic transistors has been recently proposed. In contrast to state-of-art approaches based on nano-transducers, the aforementioned single-molecule transistor (SiMoT) sensing technology is based on millimeter-sized transistors where the gate is bio-functionalized with about a trillion of antibodies. In this work we provide a detailed investigation of SiMoT biosensors by studying and quantifying the device parameters affected by the biorecognition event. The study combines both measurements and theoretical analyses, showing that a single protein detection taking place at nanometer scale affects a millimeter scale area of the biofunctionalized gate electrode. The dimension of the bioprobe domains affected by the binding is calculated as a function of the nominal protein concentration in the analyte solution. These results provide important insight into the SiMoT biosensor technology, which can guide the development of multi-modal SiMoT detection and integrated SiMoT biosensors arrays.