Nanostructured zinc oxide thin film for application to surface plasmon resonance based cholesterol biosensor

International Workshop on Thin Films for Electronics, Electro-Optics, Energy and Sensors Pub Date : 2015-11-06 DOI:10.1117/12.2199850

G. Kaur, M. Tomar, Vinay Gupta

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引用次数: 6

Abstract

ZnO thin film was deposited on gold coated glass prism by RF sputtering technique in glancing angle deposition (GLAD) configuration. The structural, morphological and optical properties of the deposited film were investigated using X-ray diffraction (XRD), Atomic Force Microscopy (AFM) and Fourier Transform Infrared (FTIR) Spectroscopy. ZnO coated Au prisms (ZnO/Au/prism) were used to excite surface plasmons in Kretschmann configuration at the Au- ZnO interface on a laboratory assembled Surface Plasmon Resonance (SPR) measurement setup. Cholesterol oxidase (ChOx) enzyme was immobilized on the ZnO/Au/prism structure by physical adsorption technique. Polydimethylsiloxane (PDMS) microchannels were fabricated over ChOx/ZnO/Au/prism system and various concentrations of cholesterol were passed over the sensor surface. The concentration of cholesterol was varied from 0.12 to 10.23 mM and the SPR reflectance curves were recorded in both static as well as dynamic modes demonstrating a high sensitivity of 0.36° mM-1.

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采用射频溅射技术，以掠角沉积(GLAD)方式在镀金玻璃棱镜上沉积ZnO薄膜。利用x射线衍射(XRD)、原子力显微镜(AFM)和傅里叶变换红外光谱(FTIR)对沉积膜的结构、形貌和光学性能进行了研究。在实验室组装的表面等离子体共振(SPR)测量装置上，利用ZnO包覆的Au棱镜(ZnO/Au/prism)在Au- ZnO界面激发Kretschmann配置的表面等离子体激元。采用物理吸附技术将胆固醇氧化酶(ChOx)固定在ZnO/Au/棱镜结构上。在ChOx/ZnO/Au/棱镜体系上制备聚二甲基硅氧烷(PDMS)微通道，并在传感器表面传递不同浓度的胆固醇。胆固醇浓度在0.12 ~ 10.23 mM范围内变化，SPR反射曲线在静态和动态模式下均有记录，灵敏度为0.36°mM-1。

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International Workshop on Thin Films for Electronics, Electro-Optics, Energy and Sensors

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