Ahmed Al-baqir, A. Hussein, I. A. Ghanem, M. Megahed
{"title":"Characterization of Paratyphoid Salmonellae Isolated from Broiler Chickens at Sharkia Governorate, Egypt","authors":"Ahmed Al-baqir, A. Hussein, I. A. Ghanem, M. Megahed","doi":"10.21608/ZVJZ.2019.10544.1028","DOIUrl":null,"url":null,"abstract":"Paratyphoid Salmonella has emerged as a global problem for humans and poultry.Therefore, in this study we investigated the occurrence, serological, antimicrobial and molecular characteristics of paratyphoid Salmonella isolated from chicken flocks at Sharkia Governorate during 2015-2016. The prevalence of paratyphoid Salmonella among the 150 suspected flocks was 32.6 % (49/150), the highest rate (41/49; 80%) was among young ages (1-10 days old) and the lowest rate (8/49; 16 %) among older ages (11- 21 days old). The highest recovery was from liver (30.66 %), followed by spleen (25.33 %), caecum (20 %) and yolk sac (15.7 %). Serotyping of 49 Salmonella isolates revealed 11 different serogroups, with Salmonella Typhimurium was the most prevalent one (24.49 %), followed by Salmonella Kentucky (18.36 %) and Salmonella Enteritidis (14.28 %). The most sensitive antibiotics were apramycin (82 %) and ciprofloxacin (65 %). Multidrug resistance (MDR) was significant to ampicillin, gentamycin and cefotriaxone in all Salmonella isolates. All phenotypically identified MDR Salmonella were found to possess invA, hilA, pefA (100%) and avrA (95 %) genes by polymerase chain reaction (PCR), confirming that these virulence genes are important virulence markers for rapid diagnosis of Salmonella infection.","PeriodicalId":137314,"journal":{"name":"Zagazig Veterinary Journal","volume":"32 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zagazig Veterinary Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21608/ZVJZ.2019.10544.1028","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3
Abstract
Paratyphoid Salmonella has emerged as a global problem for humans and poultry.Therefore, in this study we investigated the occurrence, serological, antimicrobial and molecular characteristics of paratyphoid Salmonella isolated from chicken flocks at Sharkia Governorate during 2015-2016. The prevalence of paratyphoid Salmonella among the 150 suspected flocks was 32.6 % (49/150), the highest rate (41/49; 80%) was among young ages (1-10 days old) and the lowest rate (8/49; 16 %) among older ages (11- 21 days old). The highest recovery was from liver (30.66 %), followed by spleen (25.33 %), caecum (20 %) and yolk sac (15.7 %). Serotyping of 49 Salmonella isolates revealed 11 different serogroups, with Salmonella Typhimurium was the most prevalent one (24.49 %), followed by Salmonella Kentucky (18.36 %) and Salmonella Enteritidis (14.28 %). The most sensitive antibiotics were apramycin (82 %) and ciprofloxacin (65 %). Multidrug resistance (MDR) was significant to ampicillin, gentamycin and cefotriaxone in all Salmonella isolates. All phenotypically identified MDR Salmonella were found to possess invA, hilA, pefA (100%) and avrA (95 %) genes by polymerase chain reaction (PCR), confirming that these virulence genes are important virulence markers for rapid diagnosis of Salmonella infection.