Antibacterial Efficacy and Phytochemical Screening of Ocimum gratissimum Leaf Extracts against Some Selected Bacteria Isolated from Stool Samples of Diarrhea Patients

Nigerian Journal of Technological Research Pub Date : 2021-05-24 DOI:10.4314/NJTR.V16I2.7

O. Olawale, O. Olusola-Makinde, M. Oladunmoye

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Abstract

This study investigated the antibacterial activity of Ocimum gratissimum leaf extracts on antibiotic resistant bacteria from stool samples of diarrhea patient attending specialist hospitals in Ondo State, Nigeria. O. gratissimum leaf extracted with acetone, methanol and hot water solvents were assayed for qualitative and quantitative phytochemicals. Bioactive extracts were partially purified using standard chromatographic techniques. The crude and purified acetone extract had inhibitory effect on Escherichia coli (13.00±0.58 and 26.67±0.33 mm) according to CLSI, 2014 standard. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of purified acetone extract were 12.5 and 25 mg/ml respectively. Saponin had the highest quantity (42.50±0.32 mg/ml) on acetone extract while tannin had the lowest (1.29±0.01 mg/ml). O. gratissimum leaf acetone extract could be promising candidate in the treatment of diarrhea infection which is a major concern in paediatric medicine. Keywords: Diarrhea, Ocimum gratissimum L., Antibacterial

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竹叶提取物对腹泻患者粪便中部分细菌的抑菌效果及植物化学筛选

本研究调查了在尼日利亚翁多州专科医院就诊的腹泻患者粪便样本中，八仙叶提取物对抗生素耐药菌的抑菌活性。以丙酮、甲醇和热水溶剂提取的黄菖蒲叶为研究对象，进行了植物化学成分的定性和定量分析。采用标准色谱技术对生物活性提取物进行部分纯化。丙酮粗提物和丙酮纯化物对大肠杆菌(13.00±0.58 mm和26.67±0.33 mm)的抑制作用符合CLSI 2014标准。丙酮提取物的最低抑菌浓度(MIC)为12.5 mg/ml，最低杀菌浓度(MBC)为25 mg/ml。丙酮提取物中皂苷含量最高(42.50±0.32 mg/ml)，单宁含量最低(1.29±0.01 mg/ml)。牛蒡叶丙酮提取物在治疗腹泻感染方面是一个很有前景的候选物，这是儿科医学关注的主要问题。关键词:腹泻，枸杞，抗菌

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Nigerian Journal of Technological Research

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