Automatic detection of chemotaxis cells in angiogenesis process

Abstract

A research sector mattering in the medical environment, concerns the study of the neovascularization mechanisms from pre-existing capillary vessels: Angiogenesis. These researches are very important because they correspond to many physiological but also pathological processes: in particular the growth of tumors and the development of the metastases. In this paper we are interested in the particular case of chemotaxis in the field of the angiogenesis. Indeed studies show that according to molecules applied to tumors, the capillary and other vessels develop less quickly, and can be stopped. It allows to reduce the supply to blood nutriments of the tumor and eventually to provoke its degeneration. The study proposed in this paper shows a first application on this subject with a study on in vitro cultures of umbilical cells. During a voluntary incision in a culture of blood cells, and the injection of specific molecules on this wound, we determine the healing speed. The longer the healing time is, the more effective the molecule is. The image analysis that we propose here concerns the detection of blood cells in plate and the detection of the realized incision. The created software of images treatment and the reference images are available on the following site: /www.rteam.iut-amiens.fr/angioscin/.