Evaluation of proliferation and survival of spleen immune cells treated by Deacetylchitin nanoparticles on breast cancer mouse model

Abstract

Background & Aims: Breast cancer is the most common carcinoma in women and one of the main causes of death in developed and developing countries. Today, compounds with immunolodulator properties can be replaced with routine drugs. One of them is Deacetylchitin. This study aimed to evaluate proliferation and survival of spleen immune cells treated by Deacetylchitin nanoparticles on breast cancer mouse model. Materials & Methods: Deacetylchitin nanoparticles were prepared by ionic gelation method. Zeta Sizer device measured electrical charge of nanoparticles and their size was measured by DLS and SEM. The tumor was created within two weeks after injection to BALB/c mice and then different mice groups were treated with Deacetylchitin nanoparticles and controls with PBS. After three weeks, the mice were sacrificed. The proliferation and survival of spleen lymphocyte was evaluated by MTT. Results: Deacetylchitin nanoparticles induce proliferation of spleen cells culture. Lymphocyte proliferation showed a significant increase in Deacetylchitin nanoparticles of treated group compared to control (p <0.05). Conclusion: Our findings suggest that chitosan nanoparticles can stimulate the immune system and proliferate lymphocytes. This combination can be used as a medicinal supplement to stimulate the immune system to be effective in immunotherapy.