Isolation of DNA Genomes from the Head and Middle Gland of Silkworms (Bombyx mori L)

Abstract

: The quality and quantity of DNA are very much needed for the molecular analysis of organisms. The superior quality of DNA samples can be obtained from an appropriate DNA extraction protocol.This research was conducted to isolate the DNA of silkworm larvae from the head and middle glands. The silkworms used are from three National silkworm breeding centers, namely Perhutani-Bogor, Bili-Bili and Soppeng. Organ samples taken are the head and middle silk glands. Samples are crushed and put into extraction buffers using a Mini Kit (Promega, Madison, WI, USA) in accordance with established procedures.The measurement of DNA quantity was carried out by the spectrophotometric method using a spectrophotometer at wavelengths (λ) 260 and 280 nm.DNA purity was determined by calculating the absorbance ratio at A260 to A280. DNA isolation showed a very thick band (high concentration) and also firm, this indicated that the results of DNA isolation are very good.DNA samples from the middle silk gland from the Bogor region were not obtained because the sample used when extracting was too much hence the extraction buffer used was insufficient to lyse lipids or compounds in the silkworm glands. DNA purity ranges from 1.75-2.2 with an average purity of 1.95 and the concentration of isolated DNA reaches 37.000 ng / µl.