Introduction and thesis outline

Almotasembellah Abushaban
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Abstract

Lanthanoids are gaining popularity as paramagnetic centers for high resolution nuclear magnetic resonance (NMR) spectroscopy. They provide valuable angular and long-distance restraints for structure calculations of proteins and protein complexes. The introduction of lanthanoids into a protein sample is complicated by the many requirements for functional artificial paramagnetic probes. For many applications, the probe needs to be attached site-specifically and rigidly to the protein and the coordination of the lanthanoid by the tag must result in a unique anisotropy of the magnetic susceptibility. The variety of lanthanoid coordination systems that has been explored to design paramagnetic probes that meet these demands is reviewed. Also the applications of the tags are discussed, including protein and protein complex structure determination, assignment of protein NMR spectra and the study of protein dynamics. It is concluded that lanthanoid probes bear great potential for further applications in protein NMR spectroscopy, provided that various aspects can be improved further.
引言和论文提纲
作为高分辨率核磁共振(NMR)光谱的顺磁中心,类镧越来越受欢迎。它们为蛋白质和蛋白质复合物的结构计算提供了有价值的角度和距离限制。由于对功能性人工顺磁探针的许多要求,将类镧引入蛋白质样品变得复杂。在许多应用中,探针需要位点特异性和刚性地附着在蛋白质上,而标签对类镧的配合必须导致磁化率的独特各向异性。各种镧系配位系统已经探索设计顺磁探头,以满足这些要求进行了回顾。讨论了该标签的应用,包括蛋白质和蛋白质复合物结构的测定、蛋白质核磁共振谱的赋值以及蛋白质动力学的研究。结论认为,只要各方面还有待进一步改进,类镧探针在蛋白质核磁共振光谱中具有很大的应用潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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