Evaluation of the Effect of Phytocomplex on Chondroprotective Biomarkers in an Experimental Model of Osteoarthritis in Rats

Medicina Moderna - Modern Medicine Pub Date : 2022-12-14 DOI:10.31689/rmm.2022.29.4.329

S. Pandya, Chetan Savaliya, D. Nagore, V. Undale, Shubhada Khade, Nishant Mante

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Abstract

Osteoarthritis (OA) is most prevalent joint disease and major contributor to non-fatal burden in India, with prevalence rates of 22% - 39%. The use of conventional medication can be associated with insufficient clinical management and serious side effects. The present study aims to evaluate the anti-arthritic activity and chondrocytes protection and regeneration potential of Joint Support Product (JSP) in monosodium Iodoacetate induced Osteoarthritis rat model. Pain threshold, knee joint swelling, Blood inflammatory parameters like Tissue necrosis factor, Interlukin-6, Leukotriene B 4, C- reactive protein and arthritic biomarkers Matrix metalloproteinase-13 and Cartilage Oligomeric Matrix Protein, were estimated. Also Radiographic and histopathological evaluation were done to estimate the severity of OA. Treatment of JSP demonstrated significant increase in pain threshold by 68 % and decrease in knee joint swelling by 92 %. The inflammatory markers decreased significantly (p< .00001) after treatment with JSP. The Tissue necrosis factor decreased by 55 %, Interlukin-6 by 61%, Leukotriene B 4 by 57%, C- reactive protein by 69 % and arthritic biomarkers - Matrix metalloproteinase by 88% and Cartilage Oligomeric Matrix Protein by 37% as compared to disease control rats. Also the radiological evaluation and gross histopathology of rats showed improved chondrocytes structure. Thus JSP’s analgesic, anti-inflammatory, chondrocyte regeneration, and chondroprotective properties have therefore demonstrated an anti-arthritic impact.

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植物复合物对骨关节炎大鼠实验模型中软骨保护生物标志物作用的评价

骨关节炎(OA)是印度最常见的关节疾病，也是造成非致命性负担的主要原因，患病率为22% - 39%。常规药物的使用可能与临床管理不足和严重的副作用有关。本研究旨在评价关节支撑产品(Joint Support Product, JSP)在碘乙酸钠诱导的骨关节炎大鼠模型中的抗关节炎活性及对软骨细胞的保护和再生潜力。评估疼痛阈值、膝关节肿胀、血液炎症参数如组织坏死因子、白介素-6、白三烯b4、C-反应蛋白和关节炎生物标志物基质金属蛋白酶-13和软骨寡聚基质蛋白。同时进行影像学和组织病理学评估，以评估OA的严重程度。JSP治疗显示疼痛阈值显著增加68%，膝关节肿胀减少92%。JSP治疗后炎症指标明显降低(p< 0.00001)。与疾病对照大鼠相比，组织坏死因子减少55%，白介素-6减少61%，白三烯b4减少57%，C-反应蛋白减少69%，关节炎生物标志物-基质金属蛋白酶减少88%，软骨寡聚基质蛋白减少37%。大鼠的放射学评价和大体组织病理学显示软骨细胞结构改善。因此，JSP的镇痛、抗炎、软骨细胞再生和软骨保护特性已被证明具有抗关节炎的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Medicina Moderna - Modern Medicine

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