E. R. Wikantyasning, S. D. Andasari, M. Da'i, Asyafra Nabila
{"title":"Nanoencapsulation of Zerumbone in Oleic Acid-Modified Chitosan Nanoparticles","authors":"E. R. Wikantyasning, S. D. Andasari, M. Da'i, Asyafra Nabila","doi":"10.1145/3155077.3155097","DOIUrl":null,"url":null,"abstract":"Zerumbone is an active compound of Zingiber zerumbet which has been shown to possess antioxidant and anticancer activities. The limited therapeutic application of this compound was due to its poor water solubility. The aim of this study was to encapsulate zerumbone in hydrophobic chitosan nanoparticles that modified using oleic acid. Oleic acid-modified chitosan was synthesized by coupling chitosan with oleic acid through the 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide HCl-mediated reaction, and was characterized using Fourier-transform infrared (FTIR) spectroscopy. Isolated zerumbone was coated by oleic acid-modified chitosan using ionic-gelation method. The nanoparticles was characterized using particle size analyzer for particle size and zeta potential, and the entrapment efficiency was determined by measuring the concentration of zerumbone using gas chromatography-mass spectroscopy. It was found that prepared zerumbone loaded-nanoparticles had an average size in the range from 500 to 580 nm and carried a positive charge with the zeta potential in the range of +16 to +30 mV. The entrapment efficiency of nanoparticles was about 6 times higher than encapsulation efficiency of zerumbone in chitosan nanoparticles. The nanoparticles have great potential for delivery of zerumbone as anticancer.","PeriodicalId":237079,"journal":{"name":"Proceedings of the 2017 International Conference on Computational Biology and Bioinformatics","volume":"71 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2017-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Proceedings of the 2017 International Conference on Computational Biology and Bioinformatics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1145/3155077.3155097","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
Zerumbone is an active compound of Zingiber zerumbet which has been shown to possess antioxidant and anticancer activities. The limited therapeutic application of this compound was due to its poor water solubility. The aim of this study was to encapsulate zerumbone in hydrophobic chitosan nanoparticles that modified using oleic acid. Oleic acid-modified chitosan was synthesized by coupling chitosan with oleic acid through the 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide HCl-mediated reaction, and was characterized using Fourier-transform infrared (FTIR) spectroscopy. Isolated zerumbone was coated by oleic acid-modified chitosan using ionic-gelation method. The nanoparticles was characterized using particle size analyzer for particle size and zeta potential, and the entrapment efficiency was determined by measuring the concentration of zerumbone using gas chromatography-mass spectroscopy. It was found that prepared zerumbone loaded-nanoparticles had an average size in the range from 500 to 580 nm and carried a positive charge with the zeta potential in the range of +16 to +30 mV. The entrapment efficiency of nanoparticles was about 6 times higher than encapsulation efficiency of zerumbone in chitosan nanoparticles. The nanoparticles have great potential for delivery of zerumbone as anticancer.