Visualizing dynamic processes with rapidFLIMHiRes, the ultra fast FLIM imaging method with outstanding 10ps time resolution

Abstract

Fluorescence Lifetime Imaging (FLIM) is an essential tool in Life Sciences, but up to now users had to chose between high timing precision or fast data acquisition when using Time-Correlated Single Photon Counting (TCSPC) electronics. Our approach, named rapidFLIMHiRes, allows recording several FLIM images per second with a temporal resolution of 10 ps. The method combines advances in fast scanning, hybrid photomultiplier detectors, TCSPC modules, and correction algorithms to reduce decay curve distortions. Thus fast processes can be observed with the high optical and temporal resolution achievable in confocal microscopy at a rate of several frames per second.