Characterization and Rate of Killing of Conjugated Silver Nanoparticles Against Selected Clinical Bacterial Isolates

Abstract

The menace of drug resistance, bioavailability and drug delivery to the target sites has motivated researchers to search for new antimicrobial agents from medicinal plants and subsequently use them for the biosynthesis of silver nanoparticles for effective killing of bacteria challenging to kill using crude extracts. The biosynthesis of silver nanoparticles was done using aqueous extract (AQE) of Euphorbia heterophylla, while characterization and the killing rate of conjugated silver nanoparticles (CAgNPs) were carried out using standard methods. The maximum wavelength obtained for CAgNPs was 410.33 nm, while the size distribution was 237.8 d.nm. The Fourier Transform Infra-Red result showed O-H (3308.94 cm-1), which is responsible for stabilising and reducing silver ions, while the Transmission Electron Microscopy revealed the presence of monodispersed spherical shapes CAgNPs. The Energy Dispersive Spectroscopy confirmed the presence of silver. There were reductions in the clinical bacterial isolates exposed to CAgNPs as the exposure time increased. Escherichia coli was killed between 6-7 h while Salmonella typhimurium was killed at the seven has the value of 0.00 log10 CFU/ml was recorded respectively. However, there were increments in the populations of clinical bacterial isolates in control as the time of exposure increased. Therefore, the study suggests that the CAgNPs exhibit intense antimicrobial activity and the potential to be developed as an alternative agent to treat bacterial infections, curb multidrug-resistant bacterial infection, and promote speedy drug delivery to the target sites.