Processing Condition and Properties of Protein Isolates from Mung Bean (Vigna radiata) and Quinoa (Chenopodium quinoa Willd)

Desak P. A. P. Dewi
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Abstract

Mung bean and quinoa are good sources of protein from legumes and pseudo cereals respectively. Both sources have relatively high protein content and acceptable amino acids composition. The potential and application of mung bean and quinoa as protein isolates have been discovered in some studies. Several isolation techniques such as wet fractionation (acid-base extraction), micellization, dry fractionation, aqueous separation or combination of some techniques can be used to isolate protein. This review summarized different methods and processing conditions applied during production of mung bean and quinoa protein isolates. Extracting protein from different sources may require different conditions, which results in different compositions and functional properties. Acid-base extraction is the most common method applied in mung bean and quinoa and results in high protein purity and yields. Micellization is an alternative method used in mung bean to produce higher protein content. Dry fractionation is a sustainable option used in quinoa to concentrate protein fractions. Purification methods such as ultrafiltration and aqueous phase separation can be used. Different methods and processing conditions affect functional properties, including solubility, water and oil absorption, emulsification, foaming, thermal properties of isolates, which also affect the suitable application for isolates. Keywords: Protein isolates; Quinoa; Mung bean; Wet fractionation; Dry fractionation
绿豆(Vigna radiata)和藜麦(Chenopodium Quinoa wild)蛋白分离物的加工条件及特性
绿豆和藜麦分别是豆类和伪谷物中很好的蛋白质来源。两种来源都具有相对较高的蛋白质含量和可接受的氨基酸组成。一些研究已经发现了绿豆和藜麦作为分离蛋白的潜力和应用。几种分离技术,如湿分离(酸碱萃取)、胶束、干分离、水分离或某些技术的组合,可用于分离蛋白质。本文综述了绿豆和藜麦分离蛋白生产的不同方法和加工条件。从不同来源提取蛋白质需要不同的条件,这导致蛋白质的组成和功能特性不同。酸碱萃取法是绿豆和藜麦中最常用的方法,可获得较高的蛋白质纯度和产量。胶束化是绿豆生产中提高蛋白质含量的另一种方法。干分馏是一种可持续的选择,用于藜麦浓缩蛋白质馏分。可采用超滤、水相分离等净化方法。不同的方法和加工条件会影响分离物的功能特性,包括溶解度、吸水吸油性、乳化性、起泡性、热性能等,也会影响分离物的合适应用。关键词:分离蛋白;藜麦;绿豆;湿分离;干燥的分馏
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