In vitro antioxidant and anticancer potential of Annona squamosa L. Extracts against breast cancer

Abstract

Various ailments have long been treated with plant-based remedies. This research aims to determine which compounds are present in Annona squamosa L. leaves and seeds extracts, as well as their antioxidant and anticancer potential. Annona squamosa L. hexane, methanol, and aqueous extracts were analysed for their phytochemical content, and results showed the existence of many useful compounds. The DPPH assay was used to measure the antioxidant activity. In particular, the low IC50 values revealed that the plant extracts under study possessed remarkable antioxidant activities. Antioxidant phytochemicals such alkaloid, flavonoid, phenol, oil, phytosterol, coumarin, and saponins may be responsible for these benefits. This research shows that extracts of Annona squamosa L. have a high antioxidant activity and promise as a dietary supplement and possible medicinal agent.  MTT assay was used to test for anticancer characteristics. Cell viability was assessed for each extract using a negative control and a positive control (Cisplatin). Both the Annona squamosa L. (seeds) methanolic extract and the Annona squamosa L. (leaves) water extract showed substantial efficacy in this investigation against the MCF-7 breast cancer cell line. It follows that extracts of Annona squamosa L. (leaves) and (seeds) could be used to create a natural medicine for breast cancer treatment.