Plasma irradiation effects in the abdominal adhesion mouse model

Abstract

Plasma phenomenon has been demonstrated as a new promising tool in medical field, although little is known about the plasma irradiation effects on tissues in abdominal cavity. Therefore finding the possible marker is the first and the key step to evaluate the events after plasma irradiation and a suitable mouse model is required to validate the plasma effects. We focused on abdominal adhesion formation that is the secondary event after surgery irrespective of the any surgical devices, and is associated with complications such as ileus, abdominal pain or infertility. Firstly we generated a mouse abdominal adhesion model using bipolar forceps. Within 7days, cauterized cecum was demonstrated to recruit the epididymal adipose tissue (EAT), which connected tissues abnormally and infiltrated inflammatory cells into EAT were observed by microscopic examination. We also identified some inflammatory proteins released into peritoneal cavity by proteomics of ascites after cauterization. Moreover, we measured serum cytokines level and determined that adhesion could be evaluated as a result of the alteration of pro-inflammatory and anti-inflammatory cytokines. Now we are pursuing to assess the plasma irradiation effects using those inflammatory proteins and cytokines as an appropriate marker.