Phenotyping and Genotyping of Salmonella enterica as Biofilm Producer Isolated from Diarrheal Animal and Human

Abstract

This study aimed to find out how different stress conditions (like temperature and pH) affect Salmonella enterica biofilm formation. This was done by looking at the phenotypic and genotypic features of isolates. 12 Salmonella enterica Isolate from animals, and 13 Salmonella enterica Isolate from people were used. S. enterica isolates were grown in tryptic soy broth (TSB) at (37°C, 25°C, and 42°C), and at pH levels (7, 5, and 9). T he results revealed that the percentage was 52% in the standard conditions (temperature 37ºC and pH 7) while, in another condition, observed in the same temperature (37 °C) but with pH differences (pH 5, pH 9). S. enterica , did not produce biofilm. As for the stability pH in the, pH 7 with a change in, the temperature at 25°C percentage, biofilm produce (44%) while in 42 °C (64%). The detection rates of genes, biofilm-related PCR was used to find BapA and CsgD, were 100%. Although the biofilm formation of the phenotype did not give 100% results, the genotype gave 100%, which indicates that the gene is present but not expressed. Based on the findings in this study provided valuable information on the biofilm formation of Salmonella isolated from animals and humans.