Fabrication of functional gel-nanotool for intracellular measurement

H. Maruyama, F. Arai
{"title":"Fabrication of functional gel-nanotool for intracellular measurement","authors":"H. Maruyama, F. Arai","doi":"10.1109/MHS.2009.5351873","DOIUrl":null,"url":null,"abstract":"Functional gel-nanotool impregnated with indicators for intracellular measurement in a microfluidic chip was developed. We employed membrane fusion of liposome for invasive injection of the gel-nanotool into a specific cell. The gel-nanotool is made by photo polymerization of the aggregated hydrophilic photo-crosslinkable resin. And it is modified by fluorescent dye for environment measurement. For injection to a cell, the gel-nanotool was included in fusogenic liposome. The gel-nanotool coated by fusogenic liposome was prepared by Bangham method. The gel-nanotool was manipulated by optical tweezers and contacted to the target cell. After contact of the gel-nanotool, the gel-nanotool was injected to the cell autonomously by membrane fusion of the fusogenic liposome. Injected gel-nanotool was manipulated by optical tweezers inside the cell. Detection of intracellular temperature was performed by observing the fluorescence from the gel-nanotool. We demonstrated preparation of the gel-nanotool, injection of the gel-nanotool to the cell by membrane fusion, and measurement of intracellular temperature in the chip.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"35 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"2009 International Symposium on Micro-NanoMechatronics and Human Science","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/MHS.2009.5351873","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Functional gel-nanotool impregnated with indicators for intracellular measurement in a microfluidic chip was developed. We employed membrane fusion of liposome for invasive injection of the gel-nanotool into a specific cell. The gel-nanotool is made by photo polymerization of the aggregated hydrophilic photo-crosslinkable resin. And it is modified by fluorescent dye for environment measurement. For injection to a cell, the gel-nanotool was included in fusogenic liposome. The gel-nanotool coated by fusogenic liposome was prepared by Bangham method. The gel-nanotool was manipulated by optical tweezers and contacted to the target cell. After contact of the gel-nanotool, the gel-nanotool was injected to the cell autonomously by membrane fusion of the fusogenic liposome. Injected gel-nanotool was manipulated by optical tweezers inside the cell. Detection of intracellular temperature was performed by observing the fluorescence from the gel-nanotool. We demonstrated preparation of the gel-nanotool, injection of the gel-nanotool to the cell by membrane fusion, and measurement of intracellular temperature in the chip.
细胞内测量功能凝胶-纳米工具的制备
研制了一种微流控芯片细胞内测量功能凝胶-纳米工具。我们采用脂质体膜融合技术将凝胶-纳米工具侵入性注射到特定细胞中。凝胶-纳米工具是由聚集的亲水光交联树脂光聚合制成的。并用荧光染料对其进行修饰,用于环境检测。为了注射到细胞中,凝胶-纳米工具被包含在促聚变脂质体中。采用Bangham法制备了熔融性脂质体包被凝胶纳米工具。利用光镊操纵凝胶纳米工具,使其与靶细胞接触。凝胶-纳米工具与细胞接触后,通过脂质体的膜融合将凝胶-纳米工具自动注入细胞。注入的凝胶-纳米工具由细胞内的光学镊子操纵。通过观察凝胶-纳米工具的荧光来检测细胞内温度。我们演示了凝胶纳米工具的制备,通过膜融合将凝胶纳米工具注射到细胞中,并在芯片中测量细胞内温度。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信