{"title":"Antibacterial properties of silver nanoparticles and their membranotrophic action","authors":"L. Gabrielyan, A. Trchounian","doi":"10.33581/2521-1722-2020-3-64-71","DOIUrl":null,"url":null,"abstract":"Nowadays, metal nanoparticles (NPs) can be considered as alternative agents for overcoming antibiotic resistance. The effect of silver NPs on gram-positive (Enterococcus hirae ATCC9790, Staphylococcus aureus MDC5233) and gram-negative (Escherichia coli K-12, Salmonella typhimurium MDC1759) bacteria have been investigated in this work. Silver NPs demonstrated antibacterial activity against the bacteria used, expressed in the decrease of the specific growth rate and the number of colony forming units. Moreover, the bactericidal effect of silver NPs on gram-positive bacteria was more pronounced than on gram-negative bacteria. In order to find out the mechanisms of NPs’ effects, the change of the redox potential, the hydrogen (H2) production ability, and the protons flux across the bacterial membrane have been also studied. The addition of NPs led to inhibition of H2 yield and to change of the energy-dependent proton flux through Fo F1-ATPase, indicating the silver NPs effect on the activity of membrane-bound enzymes. The data obtained point out that silver NPs show a pronounced antibacterial effect against the studied bacteria and can be used in biomedicine.","PeriodicalId":437681,"journal":{"name":"Journal of the Belarusian State University. Biology","volume":"497 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2021-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the Belarusian State University. Biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.33581/2521-1722-2020-3-64-71","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3
Abstract
Nowadays, metal nanoparticles (NPs) can be considered as alternative agents for overcoming antibiotic resistance. The effect of silver NPs on gram-positive (Enterococcus hirae ATCC9790, Staphylococcus aureus MDC5233) and gram-negative (Escherichia coli K-12, Salmonella typhimurium MDC1759) bacteria have been investigated in this work. Silver NPs demonstrated antibacterial activity against the bacteria used, expressed in the decrease of the specific growth rate and the number of colony forming units. Moreover, the bactericidal effect of silver NPs on gram-positive bacteria was more pronounced than on gram-negative bacteria. In order to find out the mechanisms of NPs’ effects, the change of the redox potential, the hydrogen (H2) production ability, and the protons flux across the bacterial membrane have been also studied. The addition of NPs led to inhibition of H2 yield and to change of the energy-dependent proton flux through Fo F1-ATPase, indicating the silver NPs effect on the activity of membrane-bound enzymes. The data obtained point out that silver NPs show a pronounced antibacterial effect against the studied bacteria and can be used in biomedicine.
目前,金属纳米颗粒(NPs)可以被认为是克服抗生素耐药性的替代药物。本文研究了银NPs对革兰氏阳性菌(希氏肠球菌ATCC9790、金黄色葡萄球菌MDC5233)和革兰氏阴性菌(大肠杆菌K-12、鼠伤寒沙门菌MDC1759)的影响。银NPs对所使用的细菌表现出抗菌活性,表现在特定生长速率和菌落形成单位数量的降低。此外,银NPs对革兰氏阳性菌的杀菌作用比革兰氏阴性菌更明显。为了找出NPs的作用机制,我们还研究了氧化还原电位、产氢能力和质子通量的变化。NPs的加入抑制了H2的产率,并改变了通过Fo f1 - atp酶的能量依赖质子通量,表明银NPs对膜结合酶的活性有影响。结果表明,银NPs对所研究的细菌具有明显的抗菌作用,可用于生物医学领域。