Fermentation pathway and redistribution of 14C in specifically labelled glucose in Cellulomonas

Zentralblatt für Bakteriologie: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie Pub Date : 1980-02-01 DOI:10.1016/S0172-5564(80)80015-7

Erko Stackebrandt , Otto Kandler

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引用次数: 7

Abstract

The distribution of ¹⁴C among and within the fermentation products formed by the fermentation of specifically labelled glucose by cellulomonades was in the main found to be compatible with a glucose breakdown via the Embden-Meyerhof-Parnas pathway. However, ¹⁴C-acetic acid labelled in both carbon atoms was formed when 3-¹⁴C- or 3.4-¹⁴C-glucose was fermented. In addition, lactic acid formed from 1-¹⁴C-, 2-¹⁴C- or 6-¹⁴C-glucose contained significant amounts of label in the carboxyl group. This non-glycolytic 14C-distribution is not caused by an additional, hitherto unknown catabolic pathway. It is, however, the result of a very effective symmetric interchange and an asymmetric redistribution of carbon atoms within the hexose molecule caused by the reversible action of aldolase, transaldolase and transketolase on sugar phosphates before their final breakdown. Up to the present, such randomization processes have been observed only in algae, plants and animal tissues.

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纤维素单胞菌特异性标记葡萄糖中14C的发酵途径和再分配

通过纤维素单糖发酵特定标记的葡萄糖形成的发酵产物之间和内部的14C分布主要与通过Embden-Meyerhof-Parnas途径的葡萄糖分解相容。然而，当3-14C-或3.4- 14c -葡萄糖发酵时，在两个碳原子上标记的14c -乙酸形成。此外，由1-14C-， 2-14C-或6- 14c -葡萄糖形成的乳酸在羧基上含有大量的标签。这种非糖酵解的14c分布不是由另一种迄今未知的分解代谢途径引起的。然而，这是一个非常有效的对称交换和碳原子在己糖分子内的不对称再分布的结果，这是由醛缩酶、转醛缩酶和转酮醇酶在磷酸糖最终分解之前对磷酸糖的可逆作用引起的。到目前为止，这种随机化过程只在藻类、植物和动物组织中观察到。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Zentralblatt für Bakteriologie: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie

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