Study on Origin traceability of Chinese Medicine Codonopsis pilosula based on stable isotope Mass Spectrometry and Machine Learning Strategy

Abstract

Origin traceability of Chinese materia medica plays an important role in ensuring the quality and efficacy of Chinese materia medica, maintaining market order and reducing the risk of medical accidents caused by counterfeit Chinese materia medica. As one of the effective techniques of origin traceability, stable isotope technology has theoretical basis and technical advantages for origin traceability of traditional Chinese medicine. In recent years, it has also been widely used in the origin traceability of some valuable traditional Chinese medicine. Discrimination of the origin of Codonopsis Radix, the results show that stable isotope carbon (δ13C and δ15N) has obvious regional characteristics, which can effectively distinguish the origin of Codonopsis Radix; and the stable isotope mass spectrometry can distinguish Codonopsis Radix from four habitats, its accuracy rate is more than 92%. Stable isotope technique combined with PCA-LDA method can effectively trace the origin of Codonopsis Radix. It aims to promote the wide application of stable isotope technology in the origin traceability of traditional Chinese medicine, and promote the establishment and improvement of stable isotope database and traceability technology system of traditional Chinese medicine.