The Impact of Adiponectin Gene Polymorphisms on the Insulin Resistance Index in Patients with Diabetes and Newly Diagnosed Type 2 Diabetes

Abstract

Background: Some adipokine hormones can affect both human and animal models of insulin resistance. Aims: This study was conducted to assess the association between rs17300539 and rs266729 of the adiponectin gene and insulin resistance and anthropometric and metabolic characteristics in impaired fasting glucose (IFG)/type 2 diabetes mellitus (T2DM) and nondiabetic participants. Methods: DNA was extracted from 80 participants with fasting blood sugar (FBS) <100 mg/dL in nondiabetics and 80 participants with FBS ≥100 mg/dL in the IFG/T2DM group. Genotyping was performed using a polymerase chain reaction-restriction fragment length polymorphism assay. Statistical analysis was performed using SPSS software version 20. Insulin and adiponectin hormone were measured using enzyme-linked immunosorbent assay and other biochemical variables were determined using the standard methods. Results: The levels of homeostatic model assessment of insulin resistance (HOMA-IR) between the IFG/T2DM and the nondiabetic group were significantly different (IFG/T2DM = 3.27, nondiabetic = 1.71; p < 0.001). The frequency of the GA genotype of rs17300539 was higher in the insulin-resistant (HOMA-IR ≥2.6, 29.7%) than in the insulin-sensitive group (HOMA-IR <2.6, 18.4%) and the GG genotype were more frequent in the insulin-sensitive group (81.6%); however, it had a marginal association (p = 0.07). This association was not statistically significant for rs266729. HOMA-IR had a positive correlation with triglyceride (TG) and total cholesterol (TC) and was negatively correlated with adiponectin level. Conclusion: IFG/T2DM patients have a higher level of HOMA-IR in comparison with nondiabetics. The genotype of GA in rs17300539 increases the risk of HOMA-IR. HOMA-IR has a positive correlation with TC and TG. Moreover, HOMA-IR increases the risk of T2DM.