Single-protein large area detections: from mechanism to applications in the clinics

Abstract

A large millimeter-wide electronic interface can detect at a single-molecule/entity limit-of-detection. The technology is called SiMoT - Single-Molecule with a large Transistor [1]. So far, antigens (Immunoglobulins, C-reactive proteins, spike 1, HIV p-24), antibodies (anti-immunoglobulins, anti-spike1), peptides, viruses (SARS-Cov-2), bacteria (Xylella fastidiosa), and even DNA strands (KRAS, miR-182) have been detected. Selectivity is assured by covering the gate electrode with a large number (1011-1012/cm2) of recognition elements to affinity binding the target element.