Optimization of Lipase-Catalyzed Asymmetric Synthesis of Ketoprofen in Non-Aqueous Media by Response Surface Methodology

Abstract

Lipase-catalyzed asymmetric synthesis of ketoprofen was developed by response surface methodology under Box-Behenkon experimental designs. The optimal reaction system was Novozym 435 from Candida Antarctica, 1 - propanol as acyl donors, isooctane as reaction medium, and water content of 0.5%. Optimization of reaction temperature, substrate molar ratio (acyl donors: ketoprofen) and enzyme concentration was carried out response surface methodology under Box-Behenkon experimental designs. Optimal conditions were: temperature 36.62 °C, substrate molar ratio 3.5:1, and enzyme concentration 4.91 g/L. The conversion was 46.68%, eep was 93.35%, and E was 73.8 at optimal conditions. Reaction progress at optimum synthesis conditions showed that very satisfactory conversion (64.74%) and production concentration (20.13 g/L) could be achieved in short time (6 h).