Molecular Diversity Analysis of Some Local Ginger (Zingiber officinale) Genotypes Using RAPD Markers

Abstract

Ginger (Zingiber officinale) rhizomes have been widely used as a spice and flavoring agent in foods and beverages in Bangladesh as well as in all over the world for its economical and medicinal values. The present investigation was undertaken for the assessment of 13 local ginger genotypes collected from different region of Bangladesh through 7 RAPD primers. Genomic DNA was extracted from ginger genotypes using CTAB method. A total of 34 distinct and differential amplification bands ranging from 150-1200 bp were observed with an average of 1.14 polymorphic bands per primer. The overall gene diversity was detected 0.8052 and the value of PIC was detected 0.7532. The RAPD marker generate enough polymorphism for possible use in diversity studies through cluster analysis and principal component analysis (PCA). PCA classified 13 ginger genotypes into four groups and showed in two dimensional scatter plot. The genetic similarity coefficients among genotypes ranged from 0.103 to 0.654. Cluster analysis based on Jaccard’s similarity-coefficient using UPGMA grouped the genotypes into two clusters: Cluster A and Cluster B. The cluster ‘A’ had only one genotype Kaptai local and the second cluster ‘B’ had rest of twelve genotypes. The prevalence level of polymorphism in the local genotypes of ginger will help to breeders for ginger improvement program. Keywords— Ginger, Molecular diversity,