{"title":"Cleavage of phosphatidylcholine: an additional mechanism for stimulation of macrophage eicosanoid synthesis?","authors":"V Kaever, H Sommermeyer, K Resch","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Cleavage of phosphatidylcholine (PtdChol) as putative mechanism leading to enhanced eicosanoid synthesis was investigated in mouse peritoneal macrophages. Addition of 12-O-tetradecanoylphorbol 13-acetate (TPA) to intact [3H]choline-labelled cells did not enhance radioactivity release above control levels. Membrane-bound PtdChol-specific phospholipase (PL) activity was then measured in a cell-free assay using [3H]choline-labelled membranes as endogenous substrate. Preincubation of macrophages with TPA increased PL activity in a time course resembling the one observed for protein kinase C (PKC) activation. Diacylglycerol (DAG) species derived from PtdChol might therefore serve as additional stimulator of rapid macrophage eicosanoid synthesis via PKC.</p>","PeriodicalId":11520,"journal":{"name":"Eicosanoids","volume":"5 Suppl ","pages":"S32-4"},"PeriodicalIF":0.0000,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Eicosanoids","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Cleavage of phosphatidylcholine (PtdChol) as putative mechanism leading to enhanced eicosanoid synthesis was investigated in mouse peritoneal macrophages. Addition of 12-O-tetradecanoylphorbol 13-acetate (TPA) to intact [3H]choline-labelled cells did not enhance radioactivity release above control levels. Membrane-bound PtdChol-specific phospholipase (PL) activity was then measured in a cell-free assay using [3H]choline-labelled membranes as endogenous substrate. Preincubation of macrophages with TPA increased PL activity in a time course resembling the one observed for protein kinase C (PKC) activation. Diacylglycerol (DAG) species derived from PtdChol might therefore serve as additional stimulator of rapid macrophage eicosanoid synthesis via PKC.
在小鼠腹膜巨噬细胞中研究了磷脂酰胆碱(PtdChol)的裂解作为导致类二十烷合成增强的推测机制。在完整的[3H]胆碱标记的细胞中添加12- o -十四烷酰磷13-醋酸酯(TPA)并没有使放射性释放增加到对照水平以上。然后使用[3H]胆碱标记的膜作为内源性底物，在无细胞试验中测量膜结合的ptdcholl特异性磷脂酶(PL)活性。巨噬细胞与TPA的预孵育增加了PL活性，其时间过程与蛋白激酶C (PKC)激活相似。因此，PtdChol衍生的二酰基甘油(DAG)可能作为巨噬细胞通过PKC快速合成类二十烷的额外刺激剂。
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