IMPACT OF ACTINOBACTERIA ON THE GROWTH AND ACCLIMATIZATION OF MICROPROPAGATED RUBUS FRUTICOSUS L. PLANTS TO EX VITRO CONDITIONS

Microbiology&Biotechnology Pub Date : 2023-05-02 DOI:10.18524/2307-4663.2023.1(57).276078

N. Tytarenko, N. Tesliuk, V. Ivanytsia

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Abstract

Microcloning is an effective method of plant reproduction that is actively developing in Ukraine for the mass propagation of valuable food crops such as blackberries. However, the problem of losing a large number of microclones often arises during the ex vitro acclimatization stage. Inoculation of the rhizosphere with potentially useful microorganisms could have a positive impact on the survival rate and biometric characteristics of acclimatized seedlings. The aim of this study was to establish the impact of 23 actinobacteria isolates on blackberry microclones (Rubus fruticosus L.) during acclimatization to ex vitro conditions, and to determine the protective potential and plant growth-promoting effects of these bacteria. Methods. The antagonistic properties of the experimental microorganisms were determined using the agar block method. Bacteria were inoculated into the rhizosphere of blackberry microclones before planting in the soil. Results. The antagonistic activity of actinobacteria against phytopathogenic fungi P. expansum, P. variotii, A. niger, C. cladosporioides, F. oxysporum, A. alternata, R. cerealis, and A. tenuissima was established. The positive effect of bacteria on micropropagated blackberry plants during acclimatization to ex vitro conditions resulted in an increase in the survival rate of microclones in the soil by 34.8%, average height of experimental plants by 2.0 cm, node number by 3.4 nodes, and leaf area by 0.4 cm2. Conclusion. It was established that isolates of mycelial actinobacteria Lim4, Myt7ch, Conc32, Conc4 were promising inoculants for ex vitro acclimatization of micropropagated plants and could be recommended for the subsequent research in order to establish the interaction mechanisms between these microorganisms and plants.

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放线菌对小苗生长和离体环境适应的影响

微克隆是一种有效的植物繁殖方法，正在乌克兰积极发展，用于黑莓等宝贵粮食作物的大规模繁殖。然而，在离体驯化阶段往往会出现大量微无性系丢失的问题。根际接种潜在有益微生物对驯化后幼苗的成活率和生物特征有积极影响。研究了23株放线菌对黑莓微无性系(Rubus fruticosus L.)离体驯化的影响，并探讨了放线菌对黑莓微无性系的保护作用和促进植物生长的作用。方法。用琼脂块法测定实验微生物的拮抗性能。在黑莓微无性系种植前，在根际接种细菌。结果。结果表明，放线菌对植物病原真菌萌发芽孢霉、变异芽孢霉、黑孢霉、枝孢霉、尖孢霉、交替芽孢霉、谷类芽孢霉和细纹霉具有拮抗作用。细菌对微繁黑莓植株在离体驯化过程中的积极作用，使其在土壤中的成活率提高34.8%，实验植株平均株高提高2.0 cm，节数增加3.4节，叶面积增加0.4 cm2。结论。结果表明，菌丝放线菌Lim4、Myt7ch、Conc32、Conc4是培养植物离体驯化的有效菌源，为进一步研究这些微生物与植物的相互作用机制提供了参考。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Microbiology&Biotechnology

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