KAJIAN KERAGAMAN GENETIK ANDALAS (MORUSMACROURA) UNTUK KONSERVASI GENETIK FLORA ASLI SUMATERA BARAT

Abstract

Conservation managementstrategies for native Sumatran species such as andalas (Morus macroura) are currently being developed through genetic diversitystudies. Theseinformationswill be used to enhance ongoing conservation and regeneration programs and to control the exploitation of these high-valueplant species. This initial study aimed to develop a method for extracting DNA from andalas cambium, to select polymorphic RAPD primers and to study genetic diversity using selected RAPD primers. DNA was successfully extracted usingthe CTAB method from all 32 cambium samples collected from Jambi and West Sumatra. Eighteen RAPD primers were screened and six polymorphic primerswereselected (OPO-6, OPY-6, OPY-15, OPW-1, OPW-3 and OPW-4). RAPD analysis using the sixselected primers resulted in 52 polymorphic loci and thevalue of genetic diversity within the population (Hs) of 0.308 and the value of genetic diversity among populations (DST) of 0.091. The highest genetic diversity was observed in Batipuah,Tanah Datar(0.351) whilethelowest was observed in Halaban,Lima Puluh Kota (0.205).A test with AMOVA shows that the genetic diversity between individuals is greater than the diversity between populations.The dendogram based on the genetic distance between populations showsthat the andalas population in West Sumatra is separated into two groups and both are separated from the Jambi population.The results of this study can be used as a basis for further research on several other andalas populations as well as the basic informationfor conservation strategiesof andalas