Efficacy of Hydroxytyrosol as Chemopreventive Agent on Induced Hamster Buccal Pouch Carcinogenesis

Abstract

ent on induced hamster buccal pouch (HBP) carcinogenesis. Subjects and Methods: Forty Syrian male hamsters five weeks old, weighing 80-120g, were divided into three groups. GI: 10 animals were left untreated. GII: 10 animals were painted with 0.5% 7, 12- dimethylbenz(a)anthracene (DMBA) in paraffin oil, 3 times a week, for 8 weeks (GIIA) and14 weeks (GIIB). GIII: chemoprevention group, 20 animals in which HT was taken at week before as well as during the application of DMBA, 3 times a week on alternative days, for 8 weeks (GIIIA) and14 weeks (GIIIB). HPB mucosa were excised and trimmed to 1-2 cm average size, then processed for hematoxylin and eosin (H&E) stain and Immunohistochemical (IHC) staining utilizing Bcl-2 and Bak antibodies. Results: Gross observation revealed variable changes in the treated (G(s)), (GII and GIII) compared to that observed in GI. Histopathological findings revealed variations among the treated groups. IHC results regarding Bcl-2 expression revealed variability in the area percentage through-out the used groups. GI, GIIA, GIIB, GIIIA and GIIIB were (6.77٪, 31.58٪, 68.37٪, 16.07٪, 48.81٪) respectively, while IHC results regarding Bak expression revealed variability in the area percentage through-out the used groups. GI, GIIA, GIIB, GIIIA and GIIIB were (45.32%, 28.58%, 10.38%, 19.32%, 38.72%) respectively. Conclusion: HT is considered as a promising chemopreventive agent in decrease the incidence of HBP carcinogenesis in gross observation as well as in histological examination.