Zinc oxide nanoparticles attenuate the oxidative damage and disturbance in antioxidant defense system induced by cyclophosphamide in male albino rats

El M Shkal Karema, Azab Azab Elsayed, Attia Ahmed M, El-Banna Sabah G, Yahya Rabia AM
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引用次数: 4

Abstract

Background: Cyclophosphamide is used for the treatment of malignant and non-malignant diseases, but, it induces oxidative damage and disturbance in the antioxidant defense system. Zinc oxide nanoparticles (ZnO NPs) are used in biomedical applications and consumer products. ZnO-NPs are protected cell membranes against oxidative damage, decrease free radicals and malondialdehyde (MDA) levels, and increase the antioxidant enzyme levels. Objectives: The present aimed to evaluate the ameliorative effect of Zn-O nano-particles on oxidative damage and disturbance in the antioxidant defense system induced by cyclophosphamide in male albino rats. Materials and Methods: 24 adult male albino rats were randomly divided into 4 groups (6 rats of each). Group I (Control group): Received 0.2 ml saline /day i.p. injection for 14 days (day by day), group II, (nZnO group): Received nZnO (5 mg/kg/day) b.w., intraperitoneally for 14 days, Group III (CP group): Received CP (20 mg/kg/day) b.w, day by day for 14 days by intraperitoneal injection, Group IV (CP + ZnO NPs group): Received nZnO group: Received nZnO (5 mg/kg/day) b.w., intraperitoneally for 14 days, plus CP (20 mg/kg/day) b.w., day by day for 14 days by intraperitoneal injection. After 24-hr from the last treatment, all animals were anesthetized using light ether. Blood, lungs, and liver samples were taken and prepared for biochemical measurements. Results: Individual treatment of zinc oxide nanoparticles and CP induced liver cytochrome b5, cytochrome C reductase, and glutathione S-transferase (GST) compared to the control group, while CP increased P450. The combination of nZnO and CP prevents the elevation of cytochrome b5, P450, cytochrome C reductase, and GST compared with the CP treated group. Zinc oxide nanoparticles and CP increased liver thiobarbituric acid reactive substances (TBARS). The combination of nZnO and CP prevents the changes in TBARS concentrations compared with the CP. Injection of CP to rats reduced the activities of serum glutathione reductase (GR) and catalase (CAT) as compared with the control group. However, combination treatment of rats with nZnO and CP increased the activities of these enzymes compared with those treated with CP alone. Zinc oxide nanoparticles and CP increased serum and lung TBARS, while decreased glutathione (GSH) concentration compared to the control group, with more pronounced changes by CP. The combination of nZnO and CP prevents the changes in TBARS and GSH concentrations compared with the CP. Conclusion: It can be concluded that CP induced oxidative stress and disturbance in the antioxidant defense system. Treatment of rats with zinc oxide nano-particles and CP together attenuated the oxidative damage and disturbance in the antioxidant defense system induced by CP. So, Patients treated with CP advised to take nZnO to prevent the side effects of chemotherapy. Further studies are necessary to evaluate the amelioration effect nZnO and other nano-particles against oxidative stress induced by CP in different doses and experimental models.
氧化锌纳米颗粒可减弱环磷酰胺引起的雄性白化大鼠氧化损伤和抗氧化防御系统紊乱
背景:环磷酰胺用于恶性和非恶性疾病的治疗,但它会引起氧化损伤和抗氧化防御系统的紊乱。氧化锌纳米颗粒(ZnO NPs)用于生物医学应用和消费品。ZnO-NPs可以保护细胞膜免受氧化损伤,降低自由基和丙二醛(MDA)水平,提高抗氧化酶水平。目的:探讨锌- o纳米颗粒对环磷酰胺诱导的雄性白化大鼠氧化损伤和抗氧化防御系统紊乱的改善作用。材料与方法:24只成年雄性白化大鼠随机分为4组,每组6只。我组(对照组):收到0.2毫升生理盐水/天腹腔注射14天(一天),第二组,(nZnO组):收到nZnO(5毫克/公斤/天)合著,腹腔内14天,第三组(CP组):收到CP(20毫克/公斤/天)合著,日复一日的14天腹腔内注射,第四组(CP +氧化锌NPs集团):收到nZnO组:收到nZnO(5毫克/公斤/天)合著,腹腔内14天,加上CP(20毫克/公斤/天)合著的,日复一日,供腹腔内注射14天。末次给药24小时后,用光醚麻醉所有动物。采集血液、肺和肝脏样本,准备进行生化测量。结果:与对照组相比,单独处理氧化锌纳米颗粒和CP可诱导肝细胞色素b5、细胞色素C还原酶和谷胱甘肽s转移酶(GST),而CP可使P450升高。与CP处理组相比,nZnO和CP联合使用可抑制细胞色素b5、P450、细胞色素C还原酶和GST的升高。氧化锌纳米颗粒和CP增加肝脏硫代巴比妥酸活性物质(TBARS)。与CP相比,nZnO和CP联合使用可抑制TBARS浓度的变化。与对照组相比,大鼠注射CP可降低血清谷胱甘肽还原酶(GR)和过氧化氢酶(CAT)活性。然而,与单独使用CP相比,nZnO和CP联合处理大鼠这些酶的活性增加。与对照组相比,氧化锌纳米颗粒和CP使血清和肺部TBARS升高,谷胱甘肽(GSH)浓度降低,且CP对TBARS和GSH浓度的影响更为明显。与CP相比,nZnO和CP联合使用可抑制TBARS和GSH浓度的变化。结论:CP可引起氧化应激和抗氧化防御系统紊乱。纳米氧化锌颗粒与CP共同治疗大鼠,可减轻CP引起的氧化损伤和抗氧化防御系统的紊乱。因此,CP治疗患者建议服用nZnO,以防止化疗的副作用。在不同剂量和实验模型下,nZnO等纳米颗粒对CP诱导的氧化应激的改善作用有待进一步研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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