Whole Body Antioxidant Status of Silver Carp Fingerlings Fed Diet Containing Various Dietary Organic Acids

Kanwal Razzaq, Tariq Dildar, Mudssar Aslam, S. Arif
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Abstract

A 10-week feeding trial was conducted to evaluate the whole- body antioxidant status of silver carp fingerlings when fed with diets containing various organic acids. Five experimental diets were formulated, such as D1 containing no supplemented organic acids, while D2 contain (2%malic acid), D3 (2%citric acid), D4 (2% formic acid) and D5 (2% lactic acid). During the experiment, water quality parameters including temperature, pH and DO were controlled. Results showed that acidification of diet significantly reduced the activity of SOD, CAT and GPX throughout the body of silver carp fingerlings. Moreover, among different organic acid groups, the maximum value was observed in citric acid while, minimum value was recorded in formic acid. Data on whole -body antioxidant enzymes were subjected to one-way analysis of variance following Steel et al. (1996). Differences between among means were compared by Tukey`s Honestly Significant Difference Test and considered significant at (p<0.05) (Snedecor and Conhran, 1991).
不同有机酸饲料对鲢鱼鱼种全身抗氧化状态的影响
通过为期10周的饲养试验,研究了不同有机酸饲料对鲢鱼幼鱼全身抗氧化能力的影响。试验配制5种试验饲粮,D1不添加有机酸,D2添加2%苹果酸、D3添加2%柠檬酸、D4添加2%甲酸、D5添加2%乳酸。实验过程中,对温度、pH、DO等水质参数进行控制。结果表明,饲料酸化显著降低了鲢鱼鱼种全身SOD、CAT和GPX的活性。不同有机酸基团中,柠檬酸含量最高,甲酸含量最低。全鱼抗氧化酶数据参照Steel et al.(1996)进行单因素方差分析。均值间的差异采用Tukey’s honest Significant Difference Test进行比较,p<0.05为显著(Snedecor and Conhran, 1991)。
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