Age-related decrease of neurogenic release of calcitonin gene-related peptide from perivascular nerves in spontaneously hypertensive rats.

Clinical and experimental hypertension. Part A, Theory and practice Pub Date : 1992-01-01 DOI:10.3109/10641969209038188

H Kawasaki, K Takasaki

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引用次数: 19

Abstract

Changes in the neurogenic release of calcitonin gene-related peptide (CGRP) by perivascular nerve stimulation (PNS) were investigated in perfused mesenteric vascular beds isolated from spontaneously hypertensive rats (SHR) and normotensive Wistar Kyoto rats (WKY). CGRP-like immunoreactivity (CGRP-LI) in the perfusate was examined by radioimmunoassay (RIA) and identified to be CGRP itself by combined analysis with radioimmunoassay and HPLC. In SHR and WKY preparations, PNS (4 and 8 Hz) evoked a frequency-dependent increase of CGRP-LI in the perfusate, which was abolished by 500 nM tetrodotoxin and the removal of Ca2+ from the medium. In young SHR and WKY (8-week-old), there was no significant difference in the release of CGRP-LI induced by PNS, whereas the release in the 15-week-old SHR was significantly less than in the 15-week-old WKY and in 8-week-old SHR. These results suggest that neural release of CGRP from perivascular nerves in mesenteric resistance vessels of SHR decreases with age.

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自发性高血压大鼠血管周围神经降钙素基因相关肽神经源性释放的年龄相关性降低。

研究了血管周围神经刺激(PNS)对自发性高血压大鼠(SHR)和正常血压Wistar Kyoto大鼠(WKY)肠系膜血管床灌注后降钙素基因相关肽(CGRP)神经源性释放的影响。用放射免疫分析法(RIA)检测灌注液中CGRP样免疫反应性(CGRP- li)，并结合放射免疫分析法和高效液相色谱法(HPLC)鉴定为CGRP本身。在SHR和WKY制剂中，PNS(4和8 Hz)引起灌注液中CGRP-LI的频率依赖性增加，这被500 nM河豚毒素和去除培养基中的Ca2+所消除。PNS诱导的CGRP-LI释放量在幼龄SHR和WKY(8周龄)中无显著差异，而15周龄SHR的CGRP-LI释放量明显低于15周龄WKY和8周龄SHR。提示SHR肠系膜阻力血管血管周围神经释放CGRP随年龄增加而减少。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Clinical and experimental hypertension. Part A, Theory and practice

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