Development and Optimization of a Surface Sterilization Protocol for the Tissue Culture of Pleurotus tuber-regium (Fr) Sing. and Auricularia auricula-judae

International Journal of Biochemistry, Bioinformatics and Biotechnology Studies Pub Date : 2022-02-15 DOI:10.37745/ijbbbs.15/vol7n2110

O. Okereke, H. Akanya, G. Ogbadu, E. Egwim, V. Etim, S. Akande

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引用次数: 1

Abstract

This study was carried out to develop and optimize a surface sterilization protocol for the sterilization of explants in the tissue culture of the edible mushrooms Pleurotus tuber-regium (Fr) Sing. and Auricularia auricula-judae. The explants were exposed to two sterilization agents; 70% ethanol (EtOH) and JIK® a locally produced bleaching solution containing 3.5% (w/v) sodium hypochlorite at concentrations of 2.5% (v/v) and 5.0% (v/v) with varying time intervals of one, two, five, ten and fifteen minutes. The results showed that treatment of explants with 5.0% (v/v) sodium hypochlorite solution for ten minutes preceded by 70% ethanol pre-treatment for two minutes gave the least explants contamination (0%) and highest explants survival (100%) for Pleurotus tuber-regium, while treatment of explants with 70% ethanol for one minute followed by 5.0% (v/v) sodium hypochlorite solution for five minutes, gave the highest survival rate (100%) and least explants contamination (0%) for Auricularia auricula-judae.

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大菱鲆组织培养表面灭菌方案的建立与优化。和黑木耳

本研究旨在建立并优化食用菌侧耳(Pleurotus tuberc -regium, Fr)组织培养外植体的表面灭菌方案。和黑木耳。外植体分别处理两种灭菌剂;70%乙醇(EtOH)和JIK®是当地生产的漂白溶液，含有3.5% (w/v)次氯酸钠，浓度为2.5% (v/v)和5.0% (v/v)，时间间隔为1、2、5、10和15分钟。结果表明:外植体用5.0% (v/v)次氯酸钠溶液处理10 min，再用70%乙醇预处理2 min，外植体污染最小(0%)，存活率最高(100%);用70%乙醇处理1 min，再用5.0% (v/v)次氯酸钠溶液处理5 min;黑木耳成活率最高(100%)，外植体污染最小(0%)。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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International Journal of Biochemistry, Bioinformatics and Biotechnology Studies

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