Comparison of Subfoveal Choroidal Thickness Measured by Two Kinds of Software

Abstract

Objective: To investigate the agreement and repeatability of choroidal thickness measurements determined by Image J software and Zeiss Cirrus HD-OCT intrinsic software. Methods: One hundred and two study participants (204 eyes) were enrolled in this research. Five line scanning by Cirrus-HD OCT with enhanced depth imaging (EDI) was performed on the area of fovea at the vertical and horizontal azimuth with scanning length of 6.0 mm. The subfoveal choroidal thickness was measured manually by Image J software and Cirrus-HD OCT intrinsic software. The agreement and repeatability of two kinds of software were described by Bland-Altman analysis, intraclass correlation coefficient (ICC) and repeatability coefficient (Rc). Results: The mean (±s) subfoveal choroidal thickness was 224.55 ± 42.91 μm for Image J software and 222.78 ± 43.80 μm for the Cirrus-HD OCT intrinsic software. The ICC of two kinds of software was 0.985 (P < 0.05), and the limits of agreement (LoA) were −13.956~17.505 μm. As compared with Cirrus-HD OCT software (11.2764 μm), the repeatability coefficient is lower calculated by Image J software (9.0694 μm). Conclusion: There was a good agreement between Image J and Cirrus-HD OCT software. The repeatability of measurement implied by Image J software was better than the Cirrus-HD OCT software. Thus, the Image J software should be used for measuring the choroidal thickness in future study.