Deneysel Otizm Spektrum Bozukluğu Modeli Oluşturulan Sıçanlarda Likopenin Beyin IL–6, IL–10, FGF–2 ve NGF Düzeyleri Üzerine Etkisi

Abstract

Rats Abstract Autism spectrum disorder (ASD) is a growing problem in western and developed civilizations. Although largely hereditary in nature, many environmental factors may play a role in triggering ASD in prone populations. Propionic acid (PPA) administration can induce serious changes including abnormal neural cell organization and subsequent autism-like neurobehavior. Since lycopene and its metabolites can be controlled in the brain, it is thought that lycopene may have neuroprotective effects on the central nervous system and may cause modulation on major brain biomarkers. In this study, 35 three-week-old Sprague Dawley male rats allocated to 5 groups: i) Control. ii) PPA; (500 mg/kg/ip). iii) PPA+LI (5 mg/kg/day intragastric lycopene given group in addition to PPA), iv) PPA+LII; (In addition to PPA, 10 mg/kg/day intragastric lycopene group), v) PPA + LIII, (20 mg/kg/day intragastric lycopene given group in addition to PPA). At the end of the study, animals were decapitated, and their brain tissues were removed and homogenized. The inflammatory cytokines interleukins 6 and 10 (IL6/IL10), basic fibroblast growth factor (FGF-2), and nerve growth factor (NGF) were obtained in the brain using SDS-PAGE and western blot techniques. Change of the expression levels was detected. According to the results, it was shown that after 35 days of administration, lycopene decreased the IL-6 and IL-10 levels due to PPA, especially in PPA + LIII and PPA + LII groups, in rats with ASD model by PPA. However, FGF-2 and NGF levels were significantly increased in all three lycopene groups compared to the PPA group (P<0.0001). In conclusion, lycopene may reduce PPA induced ASD-like neuropathological disorders by regulation of the brain inflammatory cytokines and the growth