Insilico screening of Prevotella Intermedia 17 identifies Lipopolysaccharide Biosynthesis Pathway genes as potential drug targets

2016 International Conference on Bioinformatics and Systems Biology (BSB) Pub Date : 2016-03-04 DOI:10.1109/BSB.2016.7552138

N. Mathivanan, G. Kiruthika, R. Subashree

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引用次数: 1

Abstract

Periodontal disease is an infectious disease caused by gram negative bacteria. The aim is to identify the potential drug target of Prevotella Intermedia strain 17. In silico analysis was performed to find the essential genes in Prevotella Intermedia strain 17. 470 protein sequences was retrieved from NCBI, 439 sequences passed the 90% threstold in CD-HIT, BLAST filtered 24 sequences as homolog to human and retained remaining 415 sequences. DEG identified 144 proteins as essential genes. Ezypred classified 57 essential genes as enzymes and 87 as non enzymes. Psortb identified the cellular component of the essential genes where most of the genes was found to be cytoplamic. KEGG identified the genes involved in the unique pathways of Prevotella Intermedia strain 17. Two enzymes 3-deoxy-D-manno-octulosonate cytidylyltransferase and 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase were identified to be involved in Lipopolysaccharide Biosynthesis Pathway which is a unique pathway for gram negative bacteria.

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普雷沃特菌中间体17的计算机筛选鉴定了脂多糖生物合成途径基因作为潜在的药物靶点

牙周病是一种由革兰氏阴性菌引起的传染病。目的是确定普雷沃特菌中间菌株17的潜在药物靶点。采用计算机分析方法对中间普雷沃氏菌17株进行了必要基因的筛选。从NCBI中检索到470条蛋白序列，其中439条通过CD-HIT的90%阈值，BLAST筛选了24条与人同源，保留了415条。DEG鉴定出144种蛋白质为必需基因。Ezypred将57个必需基因归为酶，87个归为非酶。Psortb鉴定了必需基因的细胞成分，其中大多数基因被发现是细胞质的。KEGG鉴定了普雷沃特菌中间菌株17的独特途径所涉及的基因。3-脱氧-d -甘露糖醛酸酯胞基转移酶和3-脱氧-d -甘露糖醛酸酯8-磷酸磷酸酶参与了革兰氏阴性菌独特的脂多糖生物合成途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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2016 International Conference on Bioinformatics and Systems Biology (BSB)

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