Histopathological analysis of the liver in hypercholesterolemia rats treated with Dillenia serrata fruits

Tri Wistya, Utami Pranita, Aritrina Laode, Kardin Sukurni, Andi Noor, Kholidha Syarifin, M. Subangkit
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Abstract

Background: Dillenia serrata (locally known as singi) is a natural product with the potential to improve liver function in hypercholesterolemia, due to anticholesterol and antioxidant properties. Objective: This study aimed to investigate the effect of D. serrata on the histopathological features of rat livers induced by a high-fat diet. Methods: This study used a quasi-experimental in vivo with a post-test-only control group design. Rats were divided into four groups: normal, high-fat diet, as well as D. serrata, and simvastatin treatment. Hypercholesterolemia was induced by a high-fat diet for two weeks. Rat liver tissues were analyzed histologically using Hematoxylin-Eosin (HE) staining, and were observed under a light microscope at 40x magnification in five wide fields of view. Results: The high-fat diet group had the most adipose cells, while the D. serrata group had the least. Statistical analysis demonstrated a significant difference between the high-fat diet group and the other groups (p < 0.05). Interestingly, no significant difference between the D. serrata group and either the normal or simvastatin group (p > 0.05), suggesting the treatment of fruit may restore liver function comparable to normal and simvastatin group. Conclusion: The D. serrata fruit reduces the number of fat cells in the histopathology of rats induced with the high-fat diet.        
枸杞子治疗高胆固醇血症大鼠肝脏组织病理学分析
背景:枸杞子是一种天然产物,由于其抗胆固醇和抗氧化特性,具有改善高胆固醇血症患者肝功能的潜力。目的:本研究旨在探讨锯齿波对高脂饮食诱导大鼠肝脏组织病理特征的影响。方法:本研究采用准实验的体内实验设计和后验对照组设计。将大鼠分为四组:正常组、高脂饮食组、D. serrata组和辛伐他汀组。高胆固醇血症是由两周的高脂肪饮食引起的。采用苏木精-伊红(HE)染色对大鼠肝脏组织进行组织学分析,并在光学显微镜下5个广角40倍放大观察。结果:高脂饮食组脂肪细胞最多,而锯齿鼠组脂肪细胞最少。经统计学分析,高脂饮食组与其他组比较差异有统计学意义(p < 0.05)。有趣的是,serrata组与正常组和辛伐他汀组之间没有显著差异(p > 0.05),表明水果治疗可能恢复肝功能与正常组和辛伐他汀组相当。结论:锯齿果可减少高脂饮食诱导大鼠组织病理学中脂肪细胞的数量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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