Analisis Hambatan Perkembangan Stadium Plasmodium Falciparum Terhadap Pemberian Ekstrak Daun Salam Badak (Acmena Acuminatissima) In Vitro

Abstract

Background: The urgency generated by drug-resistant strains of malaria parasites has accelerated the need to find a new anti-malarial drug. Recent studies have shown that 80% ethanol extract Acmena acuminatissima has suitable antimalarial activities. Aim: To analyze the inhibitory effect of A. acuminatissima extract at the developmental stage of P. falciparum in infected erythrocytes in vitro. Methods: the synchronized culture of P. falciparum was incubated along with A. acuminatissima extract, and compared with negative controls. The effect on parasite growth was determined by observing the level of parasitemia and accumulation of various infective stages seen on thin blood smears after the incubation period of 12, 24, 36, and 48 hours using a light microscope with 1000 times magnification. Results: Within 48 hours of observation the difference was mainly in the proportion of ring-stage which was 2.75% in culture with the test compound and 41.41% in the negative control which showed that ethanol extract of A. acuminatissima inhibited the growth of parasites at the schizont stage into the ring stage. In trophozoite and schizonts stages of culture with test compounds, enlargement of digestive vacuole and vesiculation is obtained. Conclusion: A. acuminatissima inhibits the development of intraerythrocyt parasites at the schizonts stage and induced morphological changes.